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KMID : 0380020070220040207
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 4 p.207 ~ p.212
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Effects of Pluronic F-68 and Oxygen Vectors on the Cell Growth of Angelica gigas Nakai in Aqueous Two-Phase System
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Jeon Su-Hwan
Lee Kyoung-Hoon
Kwon Joon-Young
Kim Dong-Il
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Abstract
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Pluronic F-68 and oxygen vectors were applied to increase the cell growth of Angelica gigas Nakai in aqueous two-phase system (ATPS). ATPS was composed of 3.6% (w/v) polyethylene glycol (PEG) 20,000 and 2.8% (w/v) crude dextran. n-Hexadecane, n-dodecane and FC-40 were used as oxygen vectors to enhance the oxygen transfer in ATPS. With 210 g/L of Pluronic F-68, addition of of n-hexadecane and FC-40 significantly enhanced the oxygen transfer rate as well as the maximum cell mass in a medium with ATPS. However, n-dodecane reduced the cell growth in all treatments. Maximum cell densities were increased up to 27.5% with 10 g/L of Pluronic F-68 and up to 40.2% with 8% (v/v) n-hexadecane compared to those of the controls without Pluronic F-68 and oxygen vectors. It was confirmed that the cell growth could be increased in ATPS using n-hexadecane.
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KEYWORD
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Pluronic F-68, oxygen vectors, aqueous two-phase system, Angelica gigas
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